Biochem/physiol Actions
Reversible: no
Target IC50: 680 nM against Cdk1
Product does not compete with ATP.
Primary Targetp34cdk1/cyclinB
Cell permeable: yes
General description
A cell-permeable and highly selective inhibitor of cyclin-dependent protein kinases (Cdks) that inhibits cell cycle progression at the G1/S and G2/M transitions. Inhibits p34cdk1/cyclinB (Cdk1; IC50 = 680 nM). Also selectively inhibits Cdk2 and Cdk5 kinases. Has little effect on casein kinase I, casein kinase II, EGF receptor kinase, MAP kinase, PKA, and PKC. Shown to prevent the phosphorylation of retinoblastoma protein and H1 histone. Also blocks Fas-induced apoptosis in HL-60 cells and shows antitumor effects on human lung cancer cell lines.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Furukawa, Y., et al. 1996. J. Biol. Chem. 271, 28469.Nishio, K., et al. 1996. Anticancer Res. 16, 3387.Kitagawa, M., et al. 1994. Oncogene 9, 2549.Kitagawa, M., et al. 1993. Oncogene 8, 2425.
Packaging
200 µg in Plastic ampoule
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Warning
Toxicity: Standard Handling (A)
This product has met the following criteria to qualify for the following awards: